Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Oxidative stress mediates end organ damage in a novel model of acetaminophen toxicity in Drosophila Scientific Reports