Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen and aspirin toxicity are both high yield, but the injury pattern is very different. Acetaminophen overdose overwhelms glutathione, allowing NAPQI to build up and cause hepatic necrosis. The antidote is N acetylcysteine, which Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Why you should avoid REGULAR acetaminophen use for babies and pregnancy 3 Little Plums